MACS/CD34 MultiSort Kit, human/130-056-701/

来自 : 发布时间：2024-05-05

Capacity: for 2×10⁹ total cells

Overview

The CD34 MultiSort Kit has been developed for the positive selection of CD34⁺ subpopulations by sequential sorting. CD34⁺ hematopoietic stem and progenitor cells can be isolated from

• peripheral blood,
• bone marrow aspirate,
• leukapheresis product,
• cord blood,
• differentiated ES and iPS cell cultures¹.

Details

Detailed separation procedure

Using the CD34 MultiSort Kit, in the first step CD34⁺ cells are positively selected after labeling with the CD34 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD34 antibody. The isolated CD34⁺ cells can be magnetically labeled and sorted according to a second antigen. The kit contains CD34 MultiSort MicroBeads, MultiSort Release Reagent, and MultiSort Stop Reagent.Control staining after separation requires a monoclonal CD34 antibody other than QBEND/10, e.g. clone AC136

Downstream applications

The CD34 MultiSort Kit is used to isolate specific subsets of CD34⁺ hematopoietic stem and progenitor cells. CD34-selected cells can be further sorted according to their expression of, e.g., HLA-DR, CD43, CD45RA, CD61, CD71, CD90 (Thy1), or CD133.

Columns

MS, LS, XS, or autoMACS^® Columns.